两种提取蒙古黄芪miRNA方法的比较

吕帝瑾, 陈婧, 钟扬, 南蓬*
复旦大学生命科学学院, 生物多样性与生态工程教育部重点实验室, 上海200433

通信作者:南蓬;E-mail: nanpeng@fudan.edu.cn;Tel: 021-65642957

摘 要:

通过对两种miRNA提取方法——一步法和多步法进行比较研究, 以期获得较高质量的蒙古黄芪不同器官的miRNA。实验结果表明, 两种方法均可用于蒙古黄芪miRNA提取, 二者存在着不同的优缺点, 多步法提取成功率较高但步骤繁琐, 相比之下一步法实验条件要求严苛但步骤简单、快捷省时, 提取的蒙古黄芪miRNA完整性好, 可以满足荧光定量PCR等进一步实验需要。

关键词:miRNA提取; 蒙古黄芪; 一步法; 多步法

收稿:2014-05-22   修定:2014-07-07

资助:国家自然科学基金(81373963和30925004)。

Compare with Two Protocols for microRNA Extraction from Astragalus membranaceus var. mongholicus

LÜ Di-Jin, CHEN Jing, ZHONG Yang, NAN Peng*
Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education, School of Life Sciences, Fudan University, Shanghai 200433, China

Corresponding author: NAN Peng; E-mail: nanpeng@fudan.edu.cn; Tel: 021-65642957

Abstract:

In order to obtain better quality miRNA in different parts of Astragalus membranaceus var. mongholicus, two miRNA extraction methods (one-step method and multi-step method) were compared. The results showed that these two methods had different advantages and disadvantages, but both of them could be used for miRNA extraction. Multi-step method had higher success rate with cumbersome steps, one-step method needed strict experimental conditions but fast and time-saving, the miRNA isolated with one-step method had high integrity which could be used for quantitative RT-PCR and so on.

Key words: miRNA extraction; Astragalus membranaceus var. mongholicus; one-step method; multi-step method

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