根据光合作用能量代谢测定光呼吸的方法研究

汪义龙, 夏莹萍, 张洁, 吴倩, 梁建生*
扬州大学生物科学与技术学院, 江苏扬州225009

通信作者:梁建生;E-mail: jsliang@yzu.edu.cn;Tel: 0514-87979320

摘 要:

本文根据光合作用和光呼吸途径能量代谢, 通过改变外界CO2和O2浓度, 计算卡尔文循环固定的CO2和光呼吸消耗的O2。结果表明, 可以通过3种方法计算。方法1, 测定在CO2饱和点(A)和正常CO2 (A`)浓度下吸收的CO2, 得出光呼吸消耗的O2为: 18/19(A–A`), 卡尔文循环固定的CO2为: 1/19(6A+13A`+19Rd)。方法2, 测定在不含O2的空气中(O)和正常O2 (O`)浓度下释放的O2, 得出光呼吸消耗的O2为: –13/5O–O`–18/5Rd, 卡尔文循环固定的CO2为: 13/18(O`–O)。方法3, 测定在正常情况下吸收的CO2 (A`)和释放的O2 (O`), 得出光呼吸消耗的O2为: 18(O`–A`), 卡尔文循环固定的CO2为: 6O`–5A`+Rd。测定在CO2饱和点和正常CO2浓度下吸收的CO2计算出水稻光呼吸释放的CO2占光合作用固定的24%~40%。

关键词:光呼吸; 光合作用; 能量代谢; 水稻

收稿:2014-03-24   修定:2014-06-24

A Method to Calculate the Photorespiration Based on Photosynthesis Energy Metabolism

WANG Yi-Yong, XIA Ying-Ping, ZHANG Jie, WU Qian, LIANG Jian-Sheng*
College of Bioscience and Biotechnology, YangzhouUniversity, Yangzhou, Jiangsu 225009, China

Corresponding author: LIANG Jian-Sheng; E-mail: jsliang@yzu.edu.cn; Tel: 0514-87979320

Abstract:

According to energy metabolism of Calvin cycle and photorespiration, the rate of CO2 fixed in Calvin cycle and O2 consumed in photorespiration were calculated by changing the external CO2 and O2 concentrations. The results showed there were three ways for calculation. Method 1, based on the measurement of CO2 absorption at CO2 saturation point (A) and normal air (A`), consumption of O2 in photorespiration could be calculated by 18/19(A–A`), and the CO2 fixed in Calvin cycle would be considered as 1/19(6A+13A`+19Rd). Method 2, on the grounds of O2 released at CO2-free air (O) and normal air (O`) conditions, consumption of O2 in photorespiration and fixation of CO2 in Calvin cycle can be deduced by formula –13/5O–O`–18/5Rd and 13/18(O`–O), respectively. Method 3, basing on the CO2 fixation (A`) and O2 consumption (O`) under the normal conditions, consumption of O2 in photorespiration and fixation of CO2 in dark reaction can be calculated as 18(O`–A`) and 6O`–5A`+Rd, respectively. According to the methods mentioned above, it was found that the ratio of CO2 released by photorespiration to photosynthesis is 24%–40% in Oryza sativa.

Key words: photorespiration; photosynthesis; energy metabolism; rice

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