文采唇柱苣苔的组织培养与快速繁殖

冼康华1,2, 付传明1, 唐凤鸾1, 石云平1, 何金祥1, 黄宁珍1,*
1广西壮族自治区中国科学院广西植物研究所, 广西桂林541006; 2广西师范大学生命科学学院, 广西桂林541001

通信作者:黄宁珍;E-mail: hnzhen68@126.com;Tel: 13807839770

摘 要:

以肉质叶为外植体, 研究文采唇柱苣苔的组织培养和快速繁殖。结果表明: 培养基MS+6-BA 0.1 mg∙L-1+NAA 0.1 mg∙L-1适用于初代培养时的愈伤组织诱导和植株再生; MS+6-BA 0.5 mg∙L-1+NAA 0.2 mg∙L-1+10%香蕉泥和MS+6-BA 0.5 mg∙L-1+NAA 0.5 mg∙L-1+10%香蕉泥分别适用于继代增殖及壮苗培养, 60 d后的增殖系数分别为6.7和4.8; 培养基MS适用于生根培养, 培养30 d, 生根率达100%。另外, 对文采唇柱苣苔生根试管苗进行大棚移栽, 移栽成活率约为94%。

关键词:文采唇柱苣苔; 肉质叶; 组织培养; 快速繁殖

收稿:2014-03-11   修定:2014-05-27

资助:国家自然科学基金项目(31160055)。

Tissue Culture and Rapid Propagation of Chirita wentsaii

XIAN Kang-Hua1,2, FU Chuan-Ming1, TANG Feng-Luan1, SHI Yun-Ping1, HE Jin-Xiang1, HUANG Ning-Zhen1,*
1Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi 541006, China; 2College of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541001, China

Corresponding author: HUANG Ning-Zhen; E-mail: hnzhen68@126.com; Tel: 13807839770

Abstract:

Tissue culture and rapid propagation of Chirita wentsaii were studied by using the fleshy leaves as explants. The results showed that the initial medium for the callus induction and seedling regeneration was MS+6-BA 0.1 mg∙L-1+NAA 0.1 mg∙L-1. The subculture media for bud proliferation and sound seedling cultivation were MS+6-BA 0.5 mg∙L-1+NAA 0.2 mg∙L-1+10% banana and MS+6-BA 0.5 mg∙L-1+NAA 0.5 mg∙L-1+ 10% banana, and their proliferation coefficients were 6.7 and 4.8 every 60 d respectively. The optimal rooting medium was MS, and the rooting rate was 100%. The rooting plantlets were transplanted in greenhouse and the highest survival rate was about 94%.

Key words: Chirita wentsaii; fleshy leaves; tissue culture; rapid propagation

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