柳杉种子无菌苗组培快繁体系的建立

蒋时姣1,2, 钟宇1, 张帆3, 刘海鹰1, 张双1,2, 吴富雨1,2, 黄金亮1,2, 许淑芬1,2, 万雪琴1,*
1四川农业大学林学院, 四川雅安625014; 2四川真美林业科技有限公司, 成都610000; 3四川农业大学风景园林学院, 成都610000

通信作者:万雪琴;E-mail: w-xue@qq.com;Tel: 0835-2882335

摘 要:

以柳杉种子在无菌条件下萌发的幼苗为外植体, 研究3种基本培养基和3种植物生长调节剂对不定芽诱导、增殖及生根的影响, 建立了完整的组培快繁体系。结果表明, 柳杉种子经预处理后, 用75%酒精消毒20 s, 再用0.1% HgCl2浸泡600 s的消毒效果较好, 污染率为10.00%, 成活率为82.33%; 丛生芽诱导的最适培养基配方为DCR+6-BA 0.2 mg•L-1, 诱导率较高, 达66.67%; 丛生芽增殖的最适培养基配方为DCR+6-BA 0.2 mg•L-1+IBA 0.05 mg•L-1, 增殖倍数达到11.00; 适宜的生根培养基为DCR+NAA 0.01 mg•L-1+IBA 0.2 mg•L-1, 生根率为90.00%, 平均生根数为4.09。

关键词:柳杉; 种子; 组织培养; 无性繁殖; 丛生芽

收稿:2014-04-22   修定:2014-05-19

资助:四川省育种攻关项目(2011NA0098-10)。

Establishment of Rapid Propagation for Sterile Seedlings of Cryptomeria fortunei by Tissue Culture

JIANG Shi-Jiao1,2, ZHONG Yu1, ZHANG Fan3, LIU Hai-Ying1, ZHANG Shuang1,2, WU Fu-Yu1,2, HUANG Jin-Liang1,2, XU Shu-Fen1,2, WAN Xue-Qin1,*
1College of Forest Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China; 2Sichuan Green Great Tree Technology, Chengdu 610000, China; 3College of Landscape Science, Sichuan Agricultural University, Chengdu 610000, China

Corresponding author: WAN Xue-Qin; E-mail: w-xue@qq.com; Tel: 0835-2882335

Abstract:

The seedlings sprouted from Cryptomeria fortunei’s seeds under sterile conditions acted as explants. The effects of 3 types of basic mediums and plant growth regulators on buds induction, multiplication and root formation were studied and the rapid propagation technique by tissue culture was established. The results showed that rapid immersed with 75% alcohol for 20 s plus 0.1% HgCl2 for 600 s was the best disinfection method for C. fortunei’s seeds after pretreatment with the contamination rate of 10.00% and the survival rate was 82.33%; the best medium for the buds induction was DCR+6-BA 0.2 mg•L-1 with the induction rate of up to 66.67%; DCR+6-BA 0.2 mg•L-1+IBA 0.05 mg•L-1 was the most suitable for buds multiplication while value ratio reaches 11.00. The optimum for rooting which rooting rate achieved 90.00% was DCR+NAA 0.01 mg•L-1+IBA 0.2 mg•L-1 and the average number of roots was 4.09.

Key words: Cryptomeria fortunei; seeds; tissue culture; asexual propagation; buds

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