拟南芥AtrbohD和AtrbohF缺失对叶蛋白质组的影响

胡盼盼, 孙立荣, 李翠, 李金侗, 郝福顺*
河南大学棉花生物学国家重点实验室, 植物逆境生物学重点实验室, 生命科学学院, 河南开封475004

通信作者:郝福顺;E-mail: haofsh@henu.edu.cn;Tel: 0371-3881387

摘 要:

拟南芥NADPH氧化酶AtrbohD和AtrbohF在脱落酸(abscisic acid, ABA)抑制主根伸长、ABA诱导气孔关闭以及植物应答干旱、盐及病菌侵染等逆境胁迫反应中发挥重要作用, 但这2个蛋白亚基缺失对拟南芥(Arabidopsis thaliana)蛋白质组的影响还未见报道。我们以营养土中生长16 d的野生型及AtrbohDAtrbohF双基因突变体atrbohD1/F1叶片为材料进行蛋白组学分析, 在双向电泳图谱上可分辨出约1 000个蛋白点, 且蛋白表达谱存在差异。选取42个显著差异蛋白点进行MALDI-TOF/TOF质谱鉴定, 成功鉴定出20个差异蛋白, 这些蛋白主要与氧化还原、能量代谢、蛋白代谢、转录和信号传导等相关, 还有一些蛋白功能未知。

关键词:拟南芥; NADPH氧化酶; 蛋白质组; 双向电泳

收稿:2014-04-24   修定:2014-05-15

资助:国家自然科学基金(31070239)。

Effects of Deficiency in AtrbohD and AtrbohF on Changes in Leaf Proteome in Arabidopsis thaliana

HU Pan-Pan, SUN Li-Rong, LI Cui, LI Jin-Tong, HAO Fu-Shun*
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, Henan 475004, China

Corresponding author: HAO Fu-Shun; E-mail: haofsh@henu.edu.cn; Tel: 0371-3881387

Abstract:

NADPH oxidase AtrbohD and AtrbohF play essential roles in abscisic acid (ABA)-inhibited primary root growth, ABA-induced stomatal closure, and in response to adverse stress conditions including disease infection, drought and salt stress in Arabidopsis. However, effects of deficiency in the two protein subunits on change in leaf proteome have not been reported. Here, leaf proteomic analysis of wild type (WT) and double mutant atrbohD1/F1 of AtrbohD and AtrbohF after grown in soil for 16 days were performed. About 1 000 protein spots in WT and the mutant were detected in the two-dimensional electrophoresis gels, and clear difference in protein profiles was observed. The 42-protein spots were selected based on their differential expression between WT and the mutant, and 20 proteins of which were identified by MALDI-TOF/TOF mass spectrometry. Most of proteins identified are involved in multiple cellular processes including redox, energy metabolism, protein metabolism, transcription and signal transduction. Some other identified proteins are functional unknown.

Key words: Arabidopsis thaliana; NADPH oxidase; proteome; two-dimensional electrophoresis

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