大薯类原球茎的离体诱导及再生体系的建立

许云, 高艳强, 高洪昌, 夏赟, 吴文嫱, 黄东益*
海南大学农学院, 海口570228

通信作者:黄东益;E-mail: dioscorea@163.com;Tel: 0898-66279037

摘 要:

采用正交试验设计方法, 以大薯带节茎段为外植体, 离体诱导类原球茎并建立大薯类原球茎的再生体系, 以解决愈伤组织分化成苗和试管苗移栽成活率低的难题。结果表明: 以带节茎段为外植体诱导类原球茎的最适培养基为MS (含3×Ca2+)+1.0 mg•L-1 6-BA+0.2 mg•L-1 NAA+0.1% PVP+3%蔗糖, 诱导率高达93.33%; 类原球茎增殖的最适培养基为MS+4 mg•L-1 6-BA+80 mg•L-1 Ad+0.1% PVP+3%蔗糖; 类原球茎生根的最适培养基: 1/2MS+0.10 mg•L-1 NAA+0.1% PVP+3%蔗糖。将诱导得到的生根类原球茎植株进行炼苗, 移栽基质珍珠岩:蛭石=2:1, 移栽成活率可达到95%。

关键词:大薯; 正交试验设计; 类原球茎

收稿:2014-04-16   修定:2014-05-20

资助:海南省重大科技项目(ZDZX2013023)、高等学校博士学科点专项科研基金(20114601110001)和海南大学地方服务项目(HDSF201304)。

In Vitro Induction and Regeneration of Protocorm-Like Bodies of Dioscorea alata

XU Yun, GAO Yan-Qiang, GAO Hong-Chang, XIA Yun, WU Wen-Qiang, HUANG Dong-Yi*
College of Agronomy, Hainan University, Haikou 570228, China

Corresponding author: HUANG Dong-Yi; E-mail: dioscorea@163.com; Tel: 0898-66279037

Abstract:

To resolve problem of low survival rate of Dioscorea alata cultured in vitro, orthogonal experimental design method was employed to induce protocorm-like body (PLB) by stem segments with axillary bud of D. alata, and establish regeneration system of PLBs. Results showed: the suitable medium for PLB induction was MS (with 3×Ca2+)+1.0 mg•L-1 6-BA+0.2 mg•L-1 NAA+0.1% PVP+3% sucrose, induction rate was 93.33%; the optimum medium for PLB multiplication was MS+4 mg•L-1 6-BA+80 mg•L-1 Ad+0.1% PVP+3% sucrose; the optimum medium for PLB roots differentiation was 1/2MS+0.10 mg•L-1 NAA+0.1% PVP+3% sucrose. Acclimatized with the ratio of perlite and vermiculite is 2:1, and after transplantation, the survival rate of rooted PLBs plantlets could reach over 95%.

Key words: Dioscorea alata; orthogonal experimental design; PLB (protocorm-like body)

此摘要已有 2246 人浏览

Back to top