转拟南芥P5CS1基因增强羽衣甘蓝的耐旱性

李鸿雁, 李大红*
黄淮学院生物工程系, 河南驻马店463000

通信作者:李大红;E-mail: lidahong27@163.com;Tel: 15036913921

摘 要:

为提高羽衣甘蓝的耐旱性, 本文将拟南芥Δ1-吡咯啉-5-羧酸合成酶(P5CS1)基因经农杆菌介导转入羽衣甘蓝植株中, 检测转基因株系与野生型植株在干旱胁迫下P5CS1 mRNA表达量、幼苗脯氨酸含量、株系根系性状、整株干重、鲜重和整株存活率。结果表明, 在15% PEG6000渗透胁迫下, 转基因植株的P5CS1基因mRNA表达量明显增加, 转基因植株脯氨酸含量是野生型的2.4倍; 主根长、最长侧根长、侧根数目、整株干重和鲜重均高于野生型, 干重/鲜重则低于野生型, 转基因植株的平均存活率为78%, 极显著高于野生型。数据显示, AtP5CS1基因在羽衣甘蓝中的表达明显改善了转基因植株的耐旱性。

关键词:Δ1-吡咯啉-5-羧酸合成酶(P5CS); 羽衣甘蓝; 脯氨酸; 耐旱性

收稿:2014-02-12   修定:2014-04-23

资助:河南省科技发展计划项目(12300410042)。

Expression of AtP5CS1 Gene Enhanced Drought Tolerance of Transgenic Brassica oleracea Plants

LI Hong-Yan, LI Da-Hong*
Department of Bioengineering, Huanghuai University, Zhumadian, Henan 463000, China

Corresponding author: LI Da-Hong; E-mail: lidahong27@163.com; Tel: 15036913921

Abstract:

To increase drought tolerance in Brassica oleracea, Δ1-pyrroline-5-carboxylate synthetase (P5CS) cDNA from Arabidopsis thaliana was transferred to B. oleracea plants mediated by agrobacterium, the P5CS1 mRNA expression, proline content, root traits, dry weight, fresh weight and whole plant survival rate of transgenic lines and wild type plants under drought stress were detected. The results showed that under 15% PEG6000 osmotic stress, the expression of P5CS1 mRNA was significantly increased. The proline content of transgenic plants was 2.4 times that of the wild type. The length of taproot, lateral root length, lateral root number, the fresh weight and dry weight of whole plant were higher than those of the wild type, and the dry weight/ fresh weight was lower than those of the wild type. The average survival of transgenic plants was 78%, significantly higher than that of wide type. These data showed AtP5CS1 gene expression in B. oleracea significantly improved drought tolerance of transgenic plants.

Key words: P5CS; Brassica oleracea; proline; drought tolerance

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