基于cDNA-AFLP技术的玉米种子劣变相关基因分析

张景龙, 杨伟飞, 吕伟增, 曹广灿, 陈军营*
河南农业大学农学院, 郑州450002

通信作者:陈军营;E-mail: chenjunying3978@126.com;Tel: 0371-63558122

摘 要:

以人工控制劣变处理(温度45 ℃, 相对湿度100%) 3 d的玉米杂交种‘郑单958’种子为材料, 通过cDNA-AFLP技术, 共获得约5 400个转录本。对差异片段进行测序分析, 最终获得122个种子劣变相关基因序列(DRS), 其中, 上调表达74个, 下调表达48个, 功能涉及初生代谢、次生代谢、蛋白代谢、转录与调控、胁迫响应、信号转导和运输。采用qRT-PCR技术分析了11个上调表达差异基因的表达模式, 结果显示不同基因的表达模式存在较大差异, 说明不同基因在种子劣变中可能扮演不同角色。

关键词:玉米种子; 人工控制劣变处理; cDNA-AFLP; 种子劣变相关基因

收稿:2014-04-08   修定:2014-06-13

资助:国家自然科学基金项目(31371709)。

Analysis of Genes Related to Seed Deterioration in Maize (Zea mays) Based on cDNA-AFLP Approach

ZHANG Jing-Long, YANG Wei-Fei, LÜ Wei-Zeng, CAO Guang-Can, CHEN Jun-Ying*
College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: CHEN Jun-Ying; E-mail: chenjunying3978@126.com; Tel: 0371-63558122

Abstract:

Hybrid seeds of maize (Zea mays) cultivar ‘Zhengdan 958’ after 3 d of controlled deterioration treatment (CDT) (temperature: 45 ℃, relative humidity: 100%) were used as the materials and about 5 400 transcript- derived fragments (TDFs) were obtained by cDNA-AFLP approach. 122 deterioration relevant sequences (DRSs) obtained by bioinformatics analysis, in which 74 were up-regulated and 48 were down-regulated, were involved in primary metabolism, secondary metabolism, protein metabolism, transcription and regulation, stress response, signal pathway and transportation. The expression patterns of 11 differential genes up-regulated were analysed by qRT-PCR and the result showed that these genes displayed different expression patterns, suggesting that they might have different roles in seed deterioration.

Key words: maize seeds; controlled deterioration treatment; cDNA-AFLP; genes related to seed deterioration

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