模拟酸雨对银杏雌雄幼苗生长及生理特性的影响

蒋雪梅1, 戚文华2, 肖娟3, 胥晓3,*
重庆三峡学院1化学与环境工程学院, 2生命科学与工程学院, 重庆404100; 3西华师范大学生命科学学院, 四川南充637009

通信作者:胥晓;E-mail: xuxiao_cwnu@163.com;Tel: 0817-2568352

摘 要:

本试验采用盆栽方法, 研究了4种pH值(5.6、5.0、4.0、3.0)条件下的模拟酸雨对银杏雌、雄幼苗生长及叶片主要生理生化特性的影响。结果表明, 随着模拟酸雨pH值的不断降低, 银杏雌、雄幼苗地上部分可见伤害逐渐出现, 其叶片相对含水量、光合色素含量和抗氧化酶活性逐渐降低, 而丙二醛、可溶性蛋白质和游离脯氨酸含量逐渐升高。与pH值为5.6的对照相比, pH值5.0和4.0的模拟酸雨对上述测定值的影响并不明显, 而在pH值3.0的模拟酸雨处理后, 大部分测定值的变化幅度达到了显著水平。尽管在上述4种处理条件下银杏雌、雄幼苗地上部分所表现出的可见伤害并无明显差异, 但从叶片相对含水量、比叶面积、抗氧化酶活性、光合色素含量和各种生化物质含量的测定值进行分析发现, 银杏雄株内部生理生化过程受到的干扰要大于雌株。由此推测, 高浓度的酸雨(pH=3.0)会影响银杏雌、雄幼苗的正常生长, 其耐酸阈值应在pH值4.0~3.0间, 且银杏雌株幼苗在耐酸性方面强于雄株。

关键词:银杏; 雌雄异株植物; 酸雨胁迫; 生长特征; 生理生化特性

收稿:2014-02-13   修定:2014-05-12

资助:西华师范大学博士科研启动基金(08B074)、重庆市科委自然科学基金(cstc2013jcyja10120)和重庆三峡学院科研创新团队(201302)。

Effects of Simulated Acid Rain on the Growth and Physiological Features of Female and Male Ginkgo biloba Seedlings

JIANG Xue-Mei1, QI Wen-Hua2, XIAO Juan3, XU Xiao3,*
1School of Chemistry and Environment Engineering, 2School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China; 3College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, China

Corresponding author: XU Xiao; E-mail: xuxiao_cwnu@163.com; Tel: 0817-2568352

Abstract:

We conducted a series of pot experiments to study the effects of simulated acid rain on the growth and physiological features of 3-year-old seedlings in female and male Ginkgo biloba. The leaves of female and male Ginkgo biloba were sprayed with simulated acid rain (pH=3.0, 4.0, or 5.0), and the treatment with pH 5.6 as the control. The results showed that under simulated acid rain stress, the aerial parts injury of female and male Ginkgo biloba seedlings gradually exposed, and leaf relative water content, photosynthetic pigment content and antioxidase activity decreased simultaneously as the pH of rainwater decreased; while the malondialdehyde, soluble protein and free proline content increased gradually. Compared with the control of pH 5.6, the above-mentioned values as affected by acid rain of pH 5.0 and 4.0 were influenced slightly, but the rangeability reached significance level under pH 3.0 rainwater treatment. There was no obvious difference in aerial parts between female and male Ginkgo biloba seedlings, but the interference of physiological and biochemical process in males suffered more severely according to the parameters of leaf relative water content, specific leaf area, photosynthetic pigment content, antioxidase activity and some biochemical materials content. So we included that higher concentration of acid rain could influence normal growth of female and male Ginkgo biloba seedlings, the rainwater of pH between 4.0 and 3.0 was the threshold for damage, moreover female seedling maybe have a stronger ability in resisting acid rain stress than male plant.

Key words: Ginkgo biloba; dioecious plant; acid rain stress; growth traits; physiological and biochemical featu

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