过量表达棉花GaSus3基因增强拟南芥的耐盐性

王敏华1,2, 丁明全2, 陈爱群3, 张毓婷1,2, 戎均康2,*
浙江农林大学1亚热带森林培育国家重点实验室培育基地, 2浙江省农产品品质改良技术研究重点实验室, 浙江临安311300; 3南京农业大学资源与环境科学学院, 南京210095

通信作者:戎均康;E-mail: jkrong@yahoo.com;Tel: 0571-63742027

摘 要:

构建了植物过量表达载体p35S::GaSus3, 通过花序浸染法成功获得转GaSus3基因拟南芥植株。利用NaCl模拟盐胁迫处理, 证实转基因拟南芥与野生型相比耐盐性明显增强。在盐胁迫下, 转基因拟南芥受到的影响较小, 而野生型则受盐害影响严重: 转基因拟南芥具有更好的萌发率和主根长度, 以保证植株正常生长; 盐胁迫下转基因拟南芥能保持较多的绿色叶片, 而野生型则过早黄化死亡。研究还发现, 转基因拟南芥的过氧化氢酶活性在胁迫前后都高于野生型, 这说明转Ga-Sus3基因能够提高拟南芥抗氧化胁迫的能力。研究结果为进一步探讨GaSus3基因在棉花耐盐方面的功能奠定了基础。

关键词:棉花GaSus3基因; 盐胁迫; 过量表达; 基因功能研究

收稿:2014-05-26   修定:2014-06-16

资助:浙江农林大学启动经费(2010FR042)。

Over Expression of a Cotton GaSus3 Gene Enhancing the Salt Stress Tolerance of Arabidopsis thaliana

WANG Min-Hua1,2, DING Ming-Quan2, CHEN Ai-Qun3, ZHANG Yu-Ting1,2, RONG Jun-Kang2,*
1Nurturing Station for the State Key Laboratory of Subtropical Silviculture, 2Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A & F University, Lin’an, Zhejiang 311300, China; 3College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: RONG Jun-Kang; E-mail: jkrong@yahoo.com; Tel: 0571-63742027

Abstract:

A plant over expression vector p35S::GaSus3 was constructed and transformed into the Arabidopsis. Transgenic plants were obtained successfully by floral dip method. Compared to the wide type plants, the salt resistance ability of GaSus3 transgenic plants were enhanced significantly under NaCl treatments. Under salt stress concentration, the growth rate of the wild-type Arabidopsis was severely suppressed, while the transgenic Arabidopsis showed less impact. Transgenic plants showed better seed germination rate and relatively longer root length than wild type, which could support plant growth under stress condition. Besides, the leaves of transgenic plants were less harmed which keep more green blade, whereas the wild-type Arabidopsis showed early yellowing and death. A relative significantly higher catalase activity had been found in transgenic plant compared with the wide type before and after salt stress treatment, which indicated that over expression of Ga- Sus3 could significantly enhance the plants ROS scavenge ability. The result showed that with the comparison of wide type plants, the salt resistance ability of transgenic plants were enhanced significantly. All these results help to establish the basis of study the functions of GaSus3 in response to salt stress in the future.

Key words: cotton GaSus3; salt stress; over expression; gene function research

此摘要已有 2365 人浏览

Back to top