锁阳种子生活力测定及前处理的方法

王进1,3, 罗光宏1,2,*, 张勇1,3, 颜霞1, 祖廷勋2,3, 张有富1
河西学院1农业与生物技术学院; 2凯源生物技术开发中心; 3甘肃省高校河西走廊特色资源利用省级重点实验室, 甘肃张掖734000

通信作者:罗光宏;E-mail: luoguanghong@163.com;Tel: 0936-8280003

摘 要:

以野生充分成熟的锁阳净种子为材料, 研究了附属物清除、硬实破除及生活力测定的方法。结果表明, 清除锁阳种子附属物最有效的方法是开水烫种, 冷至室温浸种24 h后用尼龙纱网搓揉和1%的NaOH溶液处理。破除硬实, 去除种皮最适宜的方法是1.0 mol·L-1的NaOH处理5 h、1.5 mol·L-1的NaOH处理4~5 h、2.0 mol·L-1的NaOH处理3~5 h。测定锁阳种子生活力的最佳方法是20 ℃光照条件下离体培养15 d。

关键词:锁阳种子; 生活力; 离体培养; 硬实; 附属物

收稿:2013-08-12   修定:2013-11-13

资助:甘肃省科技支撑计划(0804NKCG082)、国家自然科学基金(30960043)和甘肃省中小企业创新基金(1110-FCCG112)。

Pretreatment Methods and Determination of Seed Viability in Cynomorium songaria Rupr Seeds

WANG Jin1,3, LUO Guang-Hong1,2,*, ZHANG Yong1,3, YAN Xia1, ZU Ting-Xun2,3, ZHANG You-Fu1
1College of Agriculture and Biotechnology, 2Kaiyuan Bio-Tech Development Center, 3Microalgae Engineering Research Center of Gansu Province, Hexi University, Zhangye, Gansu 734300, China

Corresponding author: LUO Guang-Hong; E-mail: luoguanghong@163.com; Tel: 0936-8280003

Abstract:

Using wild, fully mature and wet seeds of Cynomorium songaria as materials, the different methods to remove appurtenances, break hard seedness and determinate seed viability were studied. The results showed that the most effective method to remove seeds appurtenances was boiling seeds, soaking seeds for 24 h after cooling to room temperature, and then rubbing with nylon gauze and treating with 1% NaOH solution. The most suitable method for breaking hard seeds was treating with 1 mol·L-1 NaOH for 5 h, 1.5 mol·L-1 NaOH for 4–5 h, and 2 mol·L-1 NaOH for 3–5 h. The best method to measure seed viability was culturing in vitro for 15 d under the condition of light of 20 ℃.

Key words: Cynomorium songaria; seed viability; culturing in vitro; hard seeds; appurtenances

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