濒危植物佛手参种子的非共生萌发及种苗的快速繁殖

丁兰1,*, 张丽1, 郭柳2, 桑杰2, 秦临喜2, 王保强1
1西北师范大学生命科学学院, 兰州730070; 2西藏奇正藏药股份有限公司, 西藏林芝860000

通信作者:丁兰;E-mail: dinglan@nwnu.edu.cn;Tel: 18919807050

摘 要:

离体条件下对佛手参种子进行了非共生萌发研究, 成功实现了种子萌发, 并通过根状茎增殖和分化获得了大量再生种苗。结果表明, 成熟佛手参种子难以萌发, 而未成熟种子具有萌发能力, 萌发率可达到20%, 最适萌发培养基为: 1/2MS+1.0~2.0 mg·L-1 KT+0.1 mg·L-1 NAA+10.0 mg·L-1腺嘌呤; 根状茎增殖最适培养基为: 1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+10.0 mg·L-1腺嘌呤; 芽分化最适培养基为: 1/2MS+1.0 mg·L-1 KT+10.0 mg·L-1腺嘌呤。较高浓度的细胞分裂素(1.0mg·L-1 KT)与较低浓度类生长素(0.1 mg·L-1 NAA)的配比对佛手参种子萌发、根状茎增殖及芽分化具有明显的促进作用; 较高浓度的NAA抑制种子萌发。根状茎与愈伤组织的增殖能力无明显差异, 但前者的分化能力却显著高于后者。

关键词:佛手参; 种子非共生萌发; 根状茎; 愈伤组织; 再生植株

收稿:2013-08-30   修定:2013-11-20

资助:西北师范大学知识与科技创新工程(NWNU-KJCXGC03-65)和国家自然科学基金(30960464)和生物学省级重点学科项目。

Asymbiotic Seed Germination and Rapid Seedling Regeneration of Endangered Gymnadenia conopsea (L.) R. Br.

DING Lan1,*, ZHANG Li1, GUO Liu2, SANG Jie2, QIN Lin-Xi2, WANG Bao-Qiang1
1College of Life Sciences, Northwest Normal University, Lanzhou 730070, China; 2Xizang Cheezheng Tibetan Medicine Co. Ltd, Linzhi, Tibetan 860000, China

Corresponding author: DING Lan; E-mail: dinglan@nwnu.edu.cn; Tel: 18919807050

Abstract:

In vitro asymbiotic seed germination of Gymnadenia conopsea was successfully established and a mount of regernerated seedlings were obtained via rhizomes multiplication and differentiation. The results showed that immature seeds could germinate at 20% rate while mature ones could not. The optimum germination medium was 1/2MS+1.0–2.0 mg·L-1 KT+0.1 mg·L-1 NAA+10.0 mg·L-1 adenine, the optimum media for rhizome multiplication and differentiation were 1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+10.0 mg·L-1 adenine and 1/2MS+1.0 mg·L-1 KT+10.0 mg·L-1 adenine, respectively. The combination of higher concentration of cytokinin (1.0 mg·L-1 KT) and lower concentration of auxin (0.1 mg·L-1 NAA) made promoting effect on seed germination, rhizome multiplication and bud differentiation. Higher concentration of NAA could inhibit seed germination. Rhizomes and calluses could proliferate without significant different, while the former differentiate more easily.

Key words: Gymnadenia conopsea; seed asymbiotic germination; rhizome; callus; regenerated plantlets

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