油桐叶片愈伤组织诱导及植株再生

谭晓风*, 李泽, 张琳, 龙洪旭, 袁军, 曾艳玲, 林青
经济林培育与保护教育部重点实验室(中南林业科技大学), 长沙410004

通信作者:谭晓风;E-mail: tanxiaofengcn@126.com;Tel: 0731-85623416

摘 要:

以油桐无菌苗叶片为试材, 研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明: 叶片愈伤组织的最佳诱导培养基为1/2MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 2,4-D, 诱导率达100%; 最佳分化培养基为1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA, 分化率为86.36%; 最佳继代增殖培养基为MS+3.0 mg·L-1 6-BA+0.05mg·L-1 IBA; 最佳生根培养基为1/2MS+0.1 mg·L-1 IBA, 生根率93.83%。炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中, 成活率达92%以上。

关键词:油桐; 愈伤组织; 不定芽; 植株再生

收稿:2013-07-21   修定:2013-09-23

资助:国家林业公益性行业科研专项(200904023)

Callus Induction from Leaves and Plant Regeneration of Tung Tree (Vernicia fordii Hemsley)

TAN Xiao-Feng*, LI Ze, ZHANG Lin, LONG Hong-Xu, YUAN Jun, ZENG Yan-Ling, LIN Qing
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: TAN Xiao-Feng; E-mail: tanxiaofengcn@126.com; Tel: 0731-85623416

Abstract:

The effects of different concentrations and combinations of plant growth regulators on callus induction, shoot differentiation, proliferation and root formation were studied using germ-free leaves of tung tree (Vernicia fordii) as plant materials. The results showed that the fittest medium for callus induction from leaves were 1/2MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 2,4-D, with a 100% callus induction rate; the fittest medium for shoot differentiation were 1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA, with a 86.36% differentiation rate; the best medium for shoot multiplication was MS+3.0 mg·L-1 6-BA+0.05 mg·L-1 IBA; the best rooting medium was 1/2MS+0.1 mg·L-1 IBA, with a 93.83% rooting rate. After the culture-bottle seedlings were transplanted into the matrix composed of peat soil, perlite and vermiculite (2:1:1), the survival rate was up to 92%.

Key words: Vernicia fordii; callus; adventitious shoots; plant regeneration

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