《植物生理学报》 2013, 49(11): 1245-1249
通信作者：谭晓风；E-mail: email@example.com；Tel: 0731-85623416
摘 要：以油桐无菌苗叶片为试材, 研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明: 叶片愈伤组织的最佳诱导培养基为1/2MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 2,4-D, 诱导率达100%; 最佳分化培养基为1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA, 分化率为86.36%; 最佳继代增殖培养基为MS+3.0 mg·L-1 6-BA+0.05mg·L-1 IBA; 最佳生根培养基为1/2MS+0.1 mg·L-1 IBA, 生根率93.83%。炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中, 成活率达92%以上。
关键词：油桐; 愈伤组织; 不定芽; 植株再生
Corresponding author: TAN Xiao-Feng; E-mail: firstname.lastname@example.org; Tel: 0731-85623416
Abstract:The effects of different concentrations and combinations of plant growth regulators on callus induction, shoot differentiation, proliferation and root formation were studied using germ-free leaves of tung tree (Vernicia fordii) as plant materials. The results showed that the fittest medium for callus induction from leaves were 1/2MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 2,4-D, with a 100% callus induction rate; the fittest medium for shoot differentiation were 1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA, with a 86.36% differentiation rate; the best medium for shoot multiplication was MS+3.0 mg·L-1 6-BA+0.05 mg·L-1 IBA; the best rooting medium was 1/2MS+0.1 mg·L-1 IBA, with a 93.83% rooting rate. After the culture-bottle seedlings were transplanted into the matrix composed of peat soil, perlite and vermiculite (2:1:1), the survival rate was up to 92%.
Key words: Vernicia fordii; callus; adventitious shoots; plant regeneration
此摘要已有 2002 人浏览