粉菠萝组蛋白去乙酰化酶基因AfHD1的克隆及在乙烯处理下的表达分析

李丽1, 罗轩2, 徐立1,*, 李新国2
1中国热带农业科学院热带作物品种资源研究所, 农业部华南作物基因资源与种质创制重点开放实验室, 海南儋州571737; 2海南大学园艺园林学院, 热带作物种质资源保护与开发利用教育部重点实验室, 海口570228

通信作者:徐立;E-mail: kuaifanzhongxin@126.com;Tel: 0898-23300284

摘 要:

组蛋白修饰在植物发育和防御反应中发挥着重要的作用。为研究组蛋白去乙酰化酶基因HD1的基本特征及其在乙烯调控凤梨科植物开花过程中的生物学功能, 通过筛选粉菠萝茎尖转录组测序数据并结合RACE技术分离得到AfHD1基因cDNA全长序列。该基因的开放阅读框长为1 548 bp, 编码516个氨基酸, 其理论分子量为58.28 kDa, 等电点为5.23。由该基因编码的蛋白属于组蛋白去乙酰化酶RPD3/HDA1超家族成员, 具有该家族特有的HDAC保守结构域, 该蛋白与水稻和玉米中HD1蛋白的同源性均达到87%。实时荧光定量PCR分析表明, AfHD1基因的表达受乙烯诱导, 处理后1 d的表达量与0 h相比, 提高了4倍, 推测组蛋白去乙酰化酶基因AfHD1可能与粉菠萝的乙烯信号反应有关。

关键词:粉菠萝; AfHD1; 乙烯处理; 开花

收稿:2013-08-05   修定:2013-09-22

资助:农业部热带作物种质资源利用重点开放实验室开放基金项目(KFKT-2011-06)和国家自然科学基金(31372106)。

Cloning of Histone Deacetylase AfHD1 from Aechmea fasciata and Its Expression Analysis under Ethylene Treatment

LI Li1, LUO Xuan2, XU Li1,*, LI Xin-Guo2
1Key Laboratory for Utilization of Tropical Crop Germplasm Resources, Ministry of Agriculture, Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agriculture, Danzhou, Hainan 571737, China; 2Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228, China

Corresponding author: XU Li; E-mail: kuaifanzhongxin@126.com; Tel: 0898-23300284

Abstract:

Histone modification plays an important role in plant development and plant defense responses. In order to investigate the characters of a histone deacetylase gene (HD1) and its biological functions in flowering regulation by ethylene in bromeliad, the full-length of cDNAs of AfHD1 was isolated from Aechmea fasciata by screening its shoot-tip transcriptome sequence data and a method of rapid amplification of cDNA ends (RACE). The open reading frame of AfHD1 cDNA is 1 548 bp in length, encoding 516 amino acids with molecular weight of 58.28 kDa and isoelectric point of 5.23. The putative protein of AfHD1 is a member of the RPD3/HDA1 superfamily and they have a conserved HDAC domain. Amino acid sequence alignment showed that AfHD1 shared 87% identity with HD1 homologues from rice and maize. The results of quantitative realtime PCR analysis indicated that the expression of AfHD1 could be induced by ethylene treatment and reached the peak at 1 d after processing, with the amount 4-folds higher than that at 0 h. We hypothesized that Af-HD1 may be involved in the ethylene response pathway in A. fasciata.

Key words: Aechmea fasciata; AfHD1; ethylene treatment; flowering

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