大麦氮敏感基因型苗期对氮饥饿的生理响应

徐红卫1,*, 王亦菲1,*, 刘成洪1, 陈志伟1, 杜志钊1, 高润红1, 郭桂梅1, 何婷1, 邹磊1, 卜姝明2, 黄亦辰2, 陆瑞菊1,**, 黄剑华1,**
1上海市农业科学院生物技术研究所, 上海市农业遗传育种重点实验室, 上海201106; 2上海海洋大学水产与生命学院, 上海201306

通信作者:徐红卫;E-mail: cs7@saas.sh.cn; sw1@saas.sh.cn;Tel: 021-62202965; 021-62201032

摘 要:

以大田试验获得的大麦氮敏感基因型BI-45为材料, 利用溶液培养方法, 测定了苗期株高、根长、叶绿素含量、含氮量、谷氨酰胺合成酶和硝酸还原酶活性, 以及与氮代谢相关的基因(GS1_1、GS1_2、GS1_3、GS2、Nar1、NRT2.1、NRT2.2、NRT2.3和NRT2.4)的表达。结果表明: 相对于正常供氮, 氮饥饿胁迫下, BI-45根和叶中的氮素利用率提高, 含氮量降低, 叶绿素含量减少, 根冠比增加; 叶片中的谷氨酰胺合成酶活性和硝酸还原酶的活性高于根, 但是, 与叶中的相比, 根中的谷氨酰胺合成酶活性升高及硝酸还原酶活性降低的差异性更显著; 与正常供氮相比, 氮饥饿处理下, 根中基因GS家族, 基因Nar1和硝酸盐转运蛋白基因NRT2家族的相对表达量皆达到显著性差异, 其中GS1_1、GS1_2和NRT2.2在苗期大麦氮饥饿处理下表现尤为突出, 并且在6 h都有上调表达。

关键词:氮饥饿; 氮素利用率; 谷氨酰胺合成酶; 硝酸还原酶; 硝盐酸转运蛋白

收稿:2013-10-10   修定:2013-10-29

资助:国家大麦、青稞产业技术体系(CARS-05)、上海市科委基础研究重点项目(12JC1407800)和上海市种业发展项目[沪农科种字(2012)第7号]。

The Physiological Responses of a Nitrogen Sensitive Genotype to Nitrogen

XU Hong-Wei1,*, WANG Yi-Fei1,*, LIU Cheng-Hong1, CHEN Zhi-Wei1, DU Zhi-Zhao1, GAO Run-Hong1, GUO Gui-Mei1, HE Ting1, ZOU Lei1, BU Shu-Ming2, HUANG Yi-Chen2, LU Rui-Ju1,**, HUANG Jian-Hua1,**
1Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: XU Hong-Wei; E-mail: cs7@saas.sh.cn; sw1@saas.sh.cn; Tel: 021-62202965; 021-62201032

Abstract:

In this study, using nitrogen sensitive genotype BI-45 of barley obtained from field experiments, we investigated the morphological, physiological and molecular responses, such as stem length, root length, chlorophyll content, nitrogen content, glutamine synthetase activity, nitrate reductase activity and the expression of several genes related to N uptake and assimilation. The results showed that nitrogen use efficiency and the ratios of shoot and root increase while chlorophyll and nitrogen content decrease under N starvation. Both GS and NR activities were higher in leaves than those in roots. However, compared with leaves, significant differences can be found in roots, that is, N starvation increased GS activity but decreased NR activity. In addition, genes (GS family, Nar1 and NRT2 family) in the roots also showed more significant differences, especially GS1_1, GS1_2, and NRT2.2 which were all up-regulated at 6 h might play important roles in N assimilation of barley seedlings under N starvation condition.

Key words: nitrogen starvation; nitrogen use efficiency; glutamine synthetase; nitrate reductase synthetase; nitrate transporter

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