SRK-SCR转基因拟南芥自交不亲和性的研究进展

郝艾馨, 蓝兴国, 王宇, 李玉花*
东北林业大学生命科学学院, 哈尔滨150040

通信作者:李玉花;E-mail: lyhshen@126.com;Tel: 0451-82191733

摘 要:

十字花科植物自交不亲和性(SI)受S-位点(S-locus)编码的SRK和SCR控制, 它们分别是柱头和花粉中的SI特异识别因子。野生型拟南芥不具有SI, 而近来通过转基因手段将外源SRK-SCR基因转入野生型拟南芥可以使其表现SI, 由此建立了一个可用于十字花科SI研究的新型模式植物。本文综述了利用这种转基因拟南芥在SI机制及进化方面取得的进展, 包括SI新基因的挖掘、候选基因功能分析和拟南芥生殖模式的转变等。

关键词:自交不亲和性; 拟南芥; SRK; SCR

收稿:2013-07-22   修定:2013-09-23

资助:中央高校基本科研业务费专项基金项目(DL13CA13)和国家自然科学基金项目(30900115和31070275)。

Advance in Self-Incompatibility of Arabidopsis thaliana with the Transgetic SRK-SCR

HAO Ai-Xin, LAN Xing-Guo, WANG Yu, LI Yu-Hua*
College of Life Sciences, Northeast Forestry University, Harbin 150040, China

Corresponding author: LI Yu-Hua; E-mail: lyhshen@126.com; Tel: 0451-82191733

Abstract:

In the Brassicaceae, self-incompatibility (SI) is controlled by the S-locus receptor kinase (SRK) and the S-locus cysteine-rich (SCR) protein. These two proteins are the SI specificity determinants in stigma and pollen, respectively. Recently, the Arabidopsis thaliana with SI phenotype has been achieved by transferring SRK/SCR allelic pairs from self-incompatible relative species to wild type A. thaliana. The new model plant was applied in the mechanism and evolution research of SI in Brassicaceae. This review summarizes the advances in utilizing transgenic A. thaliana SI model, including screening of new SI genes, function analysis of candidate genes and investigation of evolutionary switches to self-compatibility (SC).

Key words: self-incompatibility; Arabidopsis thaliana; SRK; SCR

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