一种植物组织原花色素显微观察的冰冻切片方法

严明理*, 刘丽莉, 王帅斌, 药园园
湖南科技大学生命科学学院, 湖南湘潭411201

通信作者:严明理;E-mail: ymljack@126.com;Tel: 0731-58291416

摘 要:

本文介绍了一种适合于植物组织原花色素观察的冰冻切片方法。采用酸性香草醛或对二甲氨基肉桂醛溶液对植物组织中的原花色素类物质进行染色, 于–80 ℃冰冻30 min后, –20 ℃下切片。这种方法能够快速观察到原花色素在细胞中的分布, 并为观察植物组织中易溶于丙酮和醇类的物质提供简便和快速的切片技术。

关键词:原花色素; 冰冻切片; 显微观察

收稿:2013-04-25   修定:2013-06-24

资助:国家自然科学基金(31000723)和湖南省普通高等学校教学改革项目(湘教通[2012]401号)。

A Suitable Cryo-Sectioning Method for Visualizing Proanthocyanidins in Plant Tissues by Light Microscopy

YAN Ming-Li*, LIU Li-Li, WANG Shuai-Bin, YAO Yuan-Yuan
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China

Corresponding author: YAN Ming-Li; E-mail: ymljack@126.com; Tel: 0731-58291416

Abstract:

This research provides a new cryo-sectioning technique for visualizing proanthocyanidins-containing elements in plant tissues. The procedure consisted of steps as follows. First, the proanthocyanidins were stained by vanillin acidic solution or p-dimethylaminocinnamaldehyde acidic solution. Then the samples were frozen at –80 ℃ for 30 min and sectioned at –20 ℃. The distribution of proanthocyanidins in cells could be quickly observed after applying this method. This described sectioning method is suitable for visualizing the substances which are more or less soluble in acetone-water and hydroalcoholic mixtures.

Key words: proanthocyanidins; cryo-sectioning; light microscopy

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