《植物生理学报》 2013, 49(8): 793-802
通信作者：朱英；E-mail: email@example.com；Tel: 0571-86419102
摘 要：为了探讨灌浆结实期高温对水稻籽粒储藏物质以及储藏物质代谢相关基因的影响。我们利用人工气候室设置高温(最高温度35 ℃/最低温度28 ℃)和常温(最高温度28 ℃/最低温度20 ℃) 2个温度处理灌浆结实期的粳稻品种Dongjin, 检测胚乳中储藏物质以及储藏物质代谢相关基因的表达。结果表明, 高温处理后水稻种子垩白明显增加、重量减小、总淀粉含量和直链淀粉含量下降。高温处理后支链淀粉链长分布也有了明显的改变, DP10~20的短链减少, 而DP>20的中长链明显增加。高温处理后淀粉合成相关基因GBSSI和BEIIb的表达量减低。大部分质体转运蛋白和淀粉合成相关基因在高温处理早期表达量增加, 而后期下降。高温处理后, 多数储藏蛋白基因如GLUB、RAG-1、RM1和PROL14表达量下降。该研究结果将有助于我们了解水稻灌浆期高温影响种子中储藏物质积累的分子机制。
关键词：高温; 水稻; 胚乳; 储藏物质
Corresponding author: ZHU Ying; E-mail: firstname.lastname@example.org; Tel: 0571-86419102
Abstract:To elucidate the effects of high temperature on grain-filling key genes involved in storage materials accumulation, a Japonica rice Dongjin was grown under high temperature (35 ℃/28 ℃) and control temperature (28 ℃/ 22 ℃) during the filling stage. The results indicated that grains ripened under high temperature resulted in the occurrence of grains with various degrees of chalky appearance, decreased grain weight and low total starch contents and amylose contents. Amylopectin chain length distribution was also significantly changed under high temperature, with smaller proportion of short side chains (DP 10–20) and larger proportion of long chains (DP>20). Several starch synthesis-related genes, such as granule-bound starch synthase I (GBSSI) and branching enzymes, especially BEIIb gene were down-regulated under high temperature. The expression levels of most of the plastid transporter protein genes and starch synthesis genes were up at early and mid-stage of high-temperature treatment, and down at late stage. The expression levels of the seed storage protein gene GLUB, RAG-1, RM1 and PROL14 were decreased under high temperature. This work helps us understand the molecular mechanism of how high temperature affecting storage material accumulation during the grain-filling stage in rice.
Key words: high temperature; rice; endosperm; storage materials
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