‘海沃德’猕猴桃叶片高频直接再生体系的建立

吴秀华, 张艳玲, 周月, 罗克明, 汤绍虎*
西南大学三峡库区生态环境教育部重点实验室, 生命科学学院, 重庆400715

通信作者:汤绍虎;E-mail: tangsh@swu.edu.cn;Tel: 13509418057

摘 要:

以‘海沃德’猕猴桃组培苗叶片为外植体, 建立叶片直接再生体系。结果表明, 叶片再生不定芽最佳培养基为MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA, 出芽率达80.95%; 不定芽增殖最佳培养基为MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1 mg·L-1 GA3, 1~3代平均增殖系数达6.38; 最适不定芽生根培养基为1/2MS+0.7 mg·L-1 IBA, 生根率为100%; 试管苗移栽成活率为92.38%。试验结果为采用叶盘法进行‘海沃德’猕猴桃的遗传转化奠定了良好基础。

关键词:‘海沃德’猕猴桃; 叶片; 高频再生体系

收稿:2013-05-15   修定:2013-07-01

资助:国家自然科学基金项目(30871576)

Establishment of High Frequency and Direct Regeneration System from Leaf of ‘Hayward’ Kiwifruit [Actinidia deliciosa (A. Chev. ) C. F. Liang et A. R. Ferguson]

WU Xiu-Hua, ZHANG Yan-Ling, ZHOU Yue, LUO Ke-Ming, TANG Shao-Hu*
Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China

Corresponding author: TANG Shao-Hu; E-mail: tangsh@swu.edu.cn; Tel: 13509418057

Abstract:

Leaves excised from ‘Hayward’ kiwifruit were used as explants to establish regeneration system. The results indicated that the maximum adventitious buds regeneration frequency achieved 80.95% on the mediu of MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA; The most suitable medium for shoot multiplication was MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1 mg·L-1 GA3 and the three generations average multiplication coefficient was up to 6.38; 1/2MS media with 0.7 mg·L-1 IBA was the most suitable for the rooting of regenerated shoots, and the rooting rate was 100%. The survival rate of hardened regenerated plantlets was as high as 92.38% after transplanting. The results laid a sound foundation for genetic transformation by means of leaf disc method.

Key words: ‘Hayward’ kiwifruit (Actinidia deliciosa); leaf; high frequency regeneration

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