《植物生理学报》 2013, 49(4): 392-399

一种高效大规模组培方法——间歇浸没培养法

许亚良, 张家明^*

中国热带农业科学院热带技术研究所, 农业部热带作物学与遗传资源利用重点实验室, 海口571101

通信作者：张家明；E-mail: jmzhang@vip.163.com；Tel: 0898-66986190

摘　要：

本文综述了间歇浸没培养法的发展, 系统介绍其对植物微繁的积极作用, 并对其目前国内外的一些应用情况作简要 介绍, 旨在增加相关领域的科研人员及企业对该方法的了解和重视, 进而加速其在国内的推广及应用, 促进相关产业的发展。

关键词：间歇浸没培养法; 微繁; 应用现状

收稿：2012-11-19   修定：2013-03-01

资助：科技部国际合作项目(2010DFA62040)和海南省农业财政专项(201301023)。

An Efficient Method for Mass Tissue Culture—Temporary Immersion Culture Principle

XU Ya-Liang, ZHANG Jia-Ming*

Key Laboratory for Tropical Crops Biology and Genetic Resources Utilization, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Corresponding author: ZHANG Jia-Ming; E-mail: jmzhang@vip.163.com; Tel: 0898-66986190

Abstract:

This review summarized the development of temporary immersion culture principle and systematically introduced it’s positive effects on plant micropropagation, and briefly introduced it’s application situation around the world, aiming at increasing the understanding and attention of related researchers and enterprises to this method, accelerating it’s application in our country, and promoting the development of related industry.

Key words: temporary immersion culture principle; micropropagation; application situation