《植物生理学报》 2013, 49(4): 351-356

集胞藻sbtA基因具有提高水稻旗叶叶绿素含量及株高的功能

杨浩萌^*, 薛哲勇, 赵乐, 漆小泉, 黄芳^*

中国科学院植物研究所, 北京100093

通信作者：杨浩萌；E-mail: yhmbjbj@ibcas.ac.cn, fhuang@ibcas.ac.cn；Tel: 010-62836546

摘　要：

SbtA是蓝藻特有的碳酸氢盐转运蛋白, 在蓝藻二氧化碳浓缩机制中起重要作用。本研究以水稻‘中花11’为材料, 采用农杆菌介导的转化方法将集胞藻的sbtA基因导入水稻胚性愈伤组织中, 再经诱导分化获得转基因幼苗。通过PCR、GFP荧光检测及Western blot检测筛选单拷贝插入的转基因阳性株系, 并对其表型进行比较分析。结果表明, 转基因水稻株高和旗叶的叶绿素含量显著提高, 与野生型相比分别增加了11.9%和27%。后一表型暗示sbtA基因可能在延缓灌浆期水稻旗叶衰老及改善水稻农艺性状方面具有潜在的应用价值。

关键词：集胞藻; sbtA; 水稻; 叶绿素含量; 株高

收稿：2013-01-05   修定：2013-02-27

资助：转基因生物新品种培育重大专项(2009ZX08009-121B)。

Increased Chlorophyll Content and Plant Height of Transgenic Rice Express-ing sbtA of Synechocystis sp. PCC 6803

YANG Hao-Meng*, XUE Zhe-Yong, ZHAO Le, QI Xiao-Quan, HUANG Fang*

Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Corresponding author: YANG Hao-Meng; E-mail: yhmbjbj@ibcas.ac.cn, fhuang@ibcas.ac.cn; Tel: 010-62836546

Abstract:

SbtA is a cyanobacterial protein involved in CO₂ concentrating mechanism (CCM). In this work, sbtA gene was cloned from Synechocystis sp. PCC6803 and introduced into rice (Oryza sativa subsp. japonica cv. ‘Zhonghua 11’) using Agrobacterium-mediated transformation. The transformants were verified by PCR and Western blot analysis. Transgenic seeds of rice were selected based on GFP reporter signal followed by pheno-typic analysis of the progeny. Our results showed that the transgenic plants were 11.9% taller than wild type. The data also showed that chlorophyll (a+b) content in the flag leaf of transgenic plants was 27% higher than that of wild-type plants. The latter phenotype observed provides a new perspective of sbtA gene towards rice improvements.

Key words: Synechocystis; sbtA; Oryza sativa; chlorophyll content; plant height