山茶‘耐冬’愈伤组织诱导与植株再生

吴斌1,2, 李纪元2, 范正琪2,*, 商宏莉1,*
1四川师范大学生命科学学院, 成都610101; 2中国林业科学研究院亚热带林业研究所, 浙江富阳311400

通信作者:范正琪;E-mail: fzq_76@126.com, honglishang0507@163.com;Tel: 0571- 63105093; 133- 88190639)。

摘 要:

以‘耐冬’子叶为外植体, 对其愈伤组织途径再生植株进行了研究。以MS基本培养基, 添加0.5 mg∙L-1 2,4-D+2.0 mg∙L-16-BA诱导愈伤组织, 诱导率达94.5%。在添加0.1 mg∙L-1 NAA和2.0 mg∙L-1 6-BA的继代培养基上经过2次转接继代培养后,将愈伤组织转入MS+0.5 mg∙L-1 NAA+10.0 mg∙L-1 6-BA或者MS+0.5 mg∙L-1 IBA+10.0 mg∙L-1 6-BA的分化培养基中, 约45 d可获得再生不定芽, 分化率约34%。最后利用“滤纸桥生根法”获得了约33%的生根山茶植株, 从而建立了‘耐冬’愈伤组织诱导及植株再生体系。

关键词:山茶; ‘耐冬’; 愈伤组织; 不定芽分化; 生根诱导; 植株再生

收稿:2012-12-26   修定:2013-01-28

资助:国家国际科技合作项目(2011DFA30490)和国家十二五科技支撑课题(2012BAD01B07)。

Callus Induction and Plant Regeneration of Camellia japonica L. ‘Naidong’

WU Bin1,2, LI Ji-Yuan2, FAN Zheng- Qi2,*, SHANG Hong- Li1,*
1College of Life Sciences, Sichuan Normal University, Chengdu, 610101, China; 2Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang 311400, China

Corresponding author: FAN Zheng- Qi; E-mail: fzq_76@126.com, honglishang0507@163.com; Tel: 0571- 63105093; 133- 88190639)。

Abstract:

Callus induction and plantlet regeneration were studied from mature cotyledons of Camellia japoni-ca L. ‘Naidong’. Up to 94.5% of the cotyledons cultured on MS medium with 0.5 mg∙L-1 2,4-D and 2.0 mg∙L-1 6-BA developed callus. The callus was then transferred to subculturing medium containing 0.1 mg∙L-1 NAA and 2.0 mg∙L-1 6-BA. Adventitious buds were obtained after 45 days on the medium with 0.5 mg∙L-1 NAA+10.0 mg∙L-1 6-BA or 0.5 mg∙L-1 IBA+10.0 mg∙L-1 6-BA, the differentiation rate was about 34%. About 33% plantes of C. japonica developed its fine root system by using “filter paper bridge rooting method”. Therefore, callus in-duction and regeneration system were achieved successfully.

Key words: Camellia japonica; ‘Naidong’; callus induction; adventitious buds differentiation; rooting induc-tion; plantlet regeneration

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