拟南芥抗旱突变体vem1对NaCl和ABA胁迫的响应

杨杰2, 江力1,*, 张娇娇1, 陈子平1
1合肥工业大学生物与食品工程学院, 合肥230009; 2合肥师范学院生命科学系, 合肥230601

通信作者:江力;E-mail: jiangli@ustc.edu.cn;Tel: 0551-62919366

摘 要:

我们从拟南芥突变体库中筛选获得一抗旱突变体vem1。本文通过观察突变体表型, 测定其脯氨酸、可溶糖、叶绿素含量和相对电导率等抗逆相关的生理指标, 研究突变体vem1对NaCl和ABA胁迫的响应。NaCl胁迫下, 突变体vem1比野生型(WT)积累更多的脯氨酸和可溶性糖, 而相对电导率、叶绿素含量均比WT低。ABA胁迫下, vem1积累的脯氨酸、可溶糖和叶绿素含量均低于WT, 而相对电导率和胁迫相关基因RD29A表达量高于WT。结果表明, vem1对NaCl胁迫具有一定的抗性, 对ABA胁迫表现敏感。研究为抗旱基因VEM1克隆及功能分析奠定基础。

关键词:拟南芥; 抗旱突变体vem1; 甘露醇; NaCl胁迫; ABA胁迫

收稿:2012-11-09   修定:2013-03-20

资助:安徽省级自然科学研究重大项目(KJ2011ZD08)和安徽省自然科学基金项目(1208085MC47)。

Responses of the Drought-Resistent vem1 Mutant to NaCl and ABA Stress in Arabidopsis

YANG Jie2, JIANG Li1,*, ZHANG Jiao-Jiao1, CHEN Zi-Ping1
1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; 2Department of Life Sci-ence, Hefei Normal University, Hefei 230601, China

Corresponding author: JIANG Li; E-mail: jiangli@ustc.edu.cn; Tel: 0551-62919366

Abstract:

We have isolated a drought-resistent mutant vem1 from the Arabidopsis mutant pool. In this work, wild-type (WT) and vem1 phenotypes were observed, and resistance-related physiological indexes, including proline content, soluble carbohydrate, chlorophyll content, and relative conductivity were measured to study re-sponse of mutant vem1 to NaCl and ABA stress. Under NaCl stress, vem1 mutant plants showed higher proline and soluble sugar levels but lower relative conductivity and chlorophyll content compared with WT plants. Un-der ABA stress, contents of proline and soluble sugar as well as chlorophyll in vem1 mutant plants were lower than those of WT plants; however, relative conductivity and the expression level of related stress-responsive gene RD29A in the vem1 mutant were higher than those in WT. These results suggest that the vem1 mutant was more resistant to NaCl stress but sensitive to ABA stress. This study provides a basis for cloning and functional analysis of the drought-resistant gene VEM1.

Key words: Arabidopsis thaliana; drought-resistent mutant vem1; mannitol; NaCl; ABA

此摘要已有 2360 人浏览

Back to top