桃PpNST1和PpSND1转录因子基因的克隆与表达分析

胡昊1,2, 刘勇1, 刘悦萍2, 邬瑞杰2, 花宝光2,*, 王有年2,*
1北京林业大学林学院, 北京100083; 2北京农学院, 农业部都市农业(北方)重点实验室, 北京102206

通信作者:花宝光;E-mail: huabg@yahoo.com, wyn1951@126.com;Tel: 010-80797225

摘 要:

为了解NAC类转录因子与桃果实内果皮发育木质化的关系, 本文以桃内果皮为试材, 采用同源基因克隆法获得了两 个NAC类转录因子的全长编码区, 分别命名为PpNST1和PpSND1。序列分析表明2个基因编码区全长均为1188 bp, 可编码396个氨基酸, 其预测蛋白均包含1个NAC结构域和2个特征结构域(LP-box和WQ-box)。氨基酸序列系统进化分析显示 PpNST1和PpSND1与苹果、葡萄、毛果杨和拟南芥等参与调控次生细胞壁形成和木质化的NST/SND1类转录因子具有较 高同源性。荧光实时定量PCR分析表明, PpNST1和PpSND1的表达水平随内果皮的木质化程度加强而不断升高, 分别于盛 花期后59和52 d达到最高表达量, 且它们在内果皮中的表达水平远高于中果皮。对PpNST1和PpSND1下游可能的作用靶标 进行进一步的表达分析表明, PpNST1和PpSND1可能在内果皮发育木质化过程中扮演重要角色。

关键词:桃; 内果皮; 发育木质化; NAC转录因子

收稿:2012-03-13   修定:2012-04-27

资助:国家自然科学基金项目(30872029)、北京市自然科学基金重点项目(6071001)、北京市自然科学基金项目 (6092007)、北京市教委重点项目(KZ201010020016)、北京市教委平台建设项目和北京市属高校人才强教深化计划项目(PHR20090516和PHR200906134)。

Cloning and Expression Analysis of PpNST1 and PpSND1 Genes from Prunus persica L.

HU Hao1,2, LIU Yong1, LIU Yue-Ping2, WU Rui-Jie2, HUA Bao-Guang2,*, WANG You-Nian2,*
1College of Forestry, Beijing Forestry University, Beijing 100083, China; 2Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China

Corresponding author: HUA Bao-Guang; E-mail: huabg@yahoo.com, wyn1951@126.com; Tel: 010-80797225

Abstract:

In order to investigate the relationship between NAC domain transcription factors and developmental lignification of peach endocarp, two NAC domain transcription factor genes PpNST1 and PpSND1 were isolated from peach (Prunus persica L. ‘Okubo’) endocarp by homologous cloning. Sequence analysis indicated that both PpNST1 and PpSND1 were 1 188 bp, encoding 396 amino acids. The predicted proteins of them contained one NAC domain in N terminus and two featured domains (LP-box and WQ-box) in C terminus. Phylogenetic analysis results revealed that PpNST1 and PpSND1 were closely related to secondary cell wall formation- and lignification-related NAC domain transcription factors in Malus domestica, Vitis vinifera, Populus trichocarpa and Arabidopsis thaliana. Quantitative real-time PCR analysis showed that the expression patterns of PpNST1 and PpSND1 were dramatically up-regulated during the lignification of peach endocarp, and their transcript expression levels were more abundant in endocarp than in mesocarp. Moreover, the expression analysis of the putative downstream targets of PpNST1 and PpSND1 suggested that they might play important role in endocarp developmental lignification.

Key words: Prunus persica; endocarp; developmental lignification; NAC domain transcription factor

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