火龙果愈伤组织诱导与植株再生

黄红梅, 任希望, 刘清波*
湖南农业大学生物科学技术学院, 长沙410128

通信作者:刘清波;E-mail: liuqingbo1740@yahoo.com.cn;Tel: 0731-84673603

摘 要:

为了建立火龙果愈伤组织诱导与植株再生体系, 以火龙果茎段、幼苗和子叶为外植体进行离体培养试验。结果表 明: 茎段诱导愈伤组织的最优培养基为1/2MS+2,4-D 2.0 mg•L-1+6-BA 0.5 mg•L-1, 诱导子叶愈伤组织的最适培养基是1/2MS+2,4-D 2.0 mg•L-1+6-BA 1.0 mg•L-1, 诱导愈伤组织分化的最优培养基为1/2MS+6-BA 4.0 mg•L-1+NAA 0.5 mg•L-1, 最佳 生根培养基为1/2MS+6-BA 1 mg•L-1+NAA 0.3 mg•L-1。

关键词:火龙果; 组织培养; 愈伤组织; 愈伤组织分化

收稿:2012-03-08   修定:2012-04-01

资助:湖南农业大学大学生创新性实验计划项目(20062886)。

Callus Induction and Plantlet Regeneration from Pitaya

HUANG Hong-Mei, REN Xi-Wang, LIU Qing-Bo*
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

Corresponding author: LIU Qing-Bo; E-mail: liuqingbo1740@yahoo.com.cn; Tel: 0731-84673603

Abstract:

To establish the system of callus induction and plantlet regeneration from pitaya, pitaya was in vitro cultured using stem segments, seedlings and cotyledons as explants. The results showed the optimum medium for the callus induction of stem segments was 1/2MS+2,4-D 2.0 mg·L-1+6-BA 0.5 mg·L-1. The most suitable medium to induce the callus from cotyledons was 1/2MS+2,4-D 2.0 mg·L-1+6-BA 1.0 mg·L-1. The optimal medium for inducing the callus differentiation was 1/2MS+6-BA 4.0 mg·L-1+NAA 0.5 mg·L-1. The greatest medium of root regeneration was 1/2MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1.

Key words: pitaya; tissue culture; callus; callus differentiation

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