《植物生理学报》 2012, 48(6): 544-548
通信作者：戴亚；E-mail: firstname.lastname@example.org;yangtiezhao@126. com；Tel: 028-86005765
摘 要：以不同烤烟品种‘红花大金元’和‘中烟100’为试验对象, 研究同一生育期不同部位叶片的无机氮积累及其与氮素代谢和氨挥发的关系。结果表明, 烟草植株下部衰老叶片(第5片叶) NO3 和NH4 的含量要高于中部叶(第10和第15片叶)和上部叶(第20片叶), 并且‘红花大金元’下部叶NO3 和NH4 的含量比‘中烟100’显著偏高; ‘中烟100’植株各部位叶片的氨气补偿点比‘红花大金元’高。两个品种在第5到第20叶位间的谷氨酰胺合成酶(GS)、硝酸还原酶(NR)和谷氨酸脱氢酶(GDH)活性大 小及其变化不一致, 是叶片无机氮积累存在品种间差异的生理基础。
关键词：烟草; 无机氮; 氨气补偿点; 氮素代谢
Corresponding author: DAI Ya; E-mail: email@example.com;yangtiezhao@126. com; Tel: 028-86005765
Abstract:Accumulation of inorganic nitrogen and its relationship with nitrogen metabolism and ammonia volatilization of different positions of leaves were investigated with different flue-cured tobacco cultivars as experimental subjects. The results indicated that the contents of NO3- and NH4+ in bottom senescent leaves (leaf 5) were higher than those in middle (leaf 10 and 15) and top leaves (leaf 20) of tobacco, and the contents of NO3- and NH4+ of ‘Honghuadajinyuan’ were significantly higher than those of ‘Zhongyan100’. And ‘Zhongyan 100’ exhibited higher apoplastic NH3 compensation points than ‘Honghuadajinyuan’. The glutamine synthetase (GS), nitrate reductase (NR) and glutamate dehydrogenase (GDH) activities and their changes in leaves of two cultivars were different. These results were physiological basis of the accumulation of inorganic nitrogen differences between cultivars.
Key words: Nicotiana tabacum; inorganic N; NH3 compensation point; nitrogen metabolism
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