拟南芥AtGLK-N基因的过量表达对代谢物质积累的影响

倪迪安*
上海应用技术学院生态技术与工程学院, 上海201418

通信作者:倪迪安;E-mail: dani@sit.edu.cn;Tel: 021-60873128

摘 要:

AtGLK-N (At2g01060)是拟南芥转录因子Golden2-like基因家族的一个成员, 仅存在于植物中。本文通过启动子-GUS 和Northern检测分析了AtGLK-N的表达模式, 结果显示: AtGLK-N在愈伤组织、球形胚、心形胚、鱼雷胚中表达, 在幼叶(真 叶)及侧根分生组织中也有表达。此外, Northern检测结果还表明AtGLK-N可被高盐胁迫所诱导。通过农杆菌介导转化拟南 芥, 得到过表达AtGLK-N的转基因植株, 其表现为矮小。为弄清AtGLK-N的功能, 对过表达AtGLK-N拟南芥进行代谢谱分析, 结果显示: 转基因植物的脯氨酸、棉籽糖和海藻糖以及催化上述代谢产物的蛋白酶的基因表达水平明显比对照植株高。 上述结果表明AtGLK-N表达主要分布在分裂细胞或组织, 并可能与高盐胁迫反应相关

关键词:AtGLK-N (At2g01060); 转录因子; 细胞分裂; 高盐诱导; 代谢谱; 表达谱

收稿:2012-01-07   修定:2012-03-28

资助:德意志学术交流中心(DAAD)特别项目。

Effect of Arabidopsis AtGLK-N Gene Over-Expressing on Metabolite Accumulation

NI Di-An*
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Corresponding author: NI Di-An; E-mail: dani@sit.edu.cn; Tel: 021-60873128

Abstract:

AtGLK-N gene (At2g01060) is a member of Arabidopsis Golden2-like family of transcription factors, which exists in plant only. The expression pattern of AtGLK-N gene was investigated by promoter-GUS system and tissue Northern analysis. The present results indicated that AtGLK-N gene expressed in leaf-derived callus, mid-globular-, heart- and torpedo-shaped embryos but not the older or younger ones. It also expressed in root tip of seedlings, new foliage leaves of seedlings, lateral root tips and meristem during all the stages of plants, suggesting that it is expressed in dividing cells. Northern analysis of Arabidopsis under stress condition indicated that AtGLK-N was induced by high salinity stress. Further more, Arabidopsis AtGLK-N over-expression lines were generated; the transgenic plants are small in size and leaf surface cells are also small compared to control. Metabolic and expression profilings were performed in leaves of Arabidopsis plants over-expressing AtGLK-N. The data showed that stress-related metabolites, such as proline, raffinose, trehalose, and the enzymes which are responsible for synthesis of above-mentioned metabolites, were increased significantly in transgenic plants over-expressing AtGLK-N compared to control. The above-mentioned data suggested that AtGLK-N expresses in cell dividing and might be involved in salinity stress response.

Key words: AtGLK-N (At2g01060); transcription factor; cell division; high-salinity stress induction; metabolic profiling; expression profiling

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